ABSTRACT
We investigated the effects of the juçara fruit (Euterpe edulis Martius) pulp and lyophilized extract on the expression of cytoprotective genes nuclear factor erythroid 2 (NF-E2)-related factor 2 (NRF2), kelch-like ECH-associated protein 1 (KEAP1), superoxide dismutase (SOD1), and glutathione peroxidase (GPX2) in human colorectal cancer cell lines (HT-29 and Caco-2). Cells were cultured for 24 h in Dulbecco's Modified Eagle's Medium containing juçara fruit pulp (5, 10, or 50 mg/mL) or lyophilized extract (0.05, 0.1, or 0.5 mg/mL), and gene expression was quantified using real-time quantitative reverse transcription polymerase chain reaction. All studied genes showed significant variation in gene expression among different concentrations of pulp or lyophilized extract. Overall, the expression of the selected genes decreased in both cell lines following exposure to the pulp or lyophilized extract in a dose-dependent manner for most of the concentrations studied. In summary, our study showed that the compounds in juçara fruit inhibited the expression of cytoprotective genes associated with the antioxidant response and that, although not cytotoxic at the concentrations studied, they could potentially block the activation of the NRF2/KEAP1 pathway.
ABSTRACT
O presente experimento foi conduzido com o objetivo de avaliar a substituição parcial da proteína bruta (PB) do feno da alfafa (FA) pela PB do feno de maniçoba (FM) na alimentação de coelhos em crescimento, bem como o valor nutricional da proteína bruta do feno de maniçoba. Foram estudados os parâmetros de desempenho, digestibilidade, rendimento de carcaça e dos cortes nobres submetidos às dietas experimentais. Os tratamentos consistiram em quatro níveis de substituição (0, 25, 50 e 75%) da proteína do feno de alfafa pelo feno de maniçoba. A substituição crescente dos níveis de feno maniçoba resultou em um aumento linear no consumo de ração e no ganho de peso de forma quadrática aos 83 dias, mostrando que esse ingrediente pode ser utilizado como substituto ao feno de alfafa na dieta de coelhos. A proteína do feno da alfafa pode ser substituída parcialmente pela proteína do feno de maniçoba.
The experiment was conducted to evaluate the nutritional value and the partial substitution of crude protein (CP) of alfalfa hay (FA) with CP hay maniçoba (FM) in diets for growing rabbits. The performance, digestibility, carcass yield and prime cuts parameters submitted to experimental diets were studied. Treatments consisted of four levels (0, 25, 50 and 75%) of protein alfalfa hay and maniçoba hay. The increasing substitution levels of maniçoba hay resulted in a linear increase in feed intake and weight gain quadratically at 83 days, showing that this ingredient can be used as a substitute for alfalfa hay in the diet of rabbits. The protein of alfalfa hay can be partially replaced by the maniçoba protein hay.